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Image Search Results
Journal:
Article Title: The Lantibiotic Mersacidin Inhibits Peptidoglycan Synthesis by Targeting Lipid II
doi:
Figure Lengend Snippet: (A and B) Interaction of mersacidin with isolated lipid II. Various amounts of [14C]lipid II and either [14C]mersacidin or unlabeled mersacidin were incubated and subjected to PAGE under nondenaturing conditions (A) or in the presence of 1% SDS in the sample buffer and 0.1% SDS in the running buffer (B). Lane 1, 0.4 nmol of [14C]mersacidin and 0.4 nmol of [14C]lipid II; lane 2, 0.8 nmol of [14C]mersacidin and 0.4 nmol of [14C]lipid II; lane 3, 0.4 nmol of [14C]mersacidin and 0.8 nmol of [14C]lipid II; lane 4, 4 nmol of unlabeled mersacidin and 0.4 nmol of [14C]lipid II; lane 5, 0.4 nmol of [14C]mersacidin; lane 6, 0.4 nmol of [14C]lipid II. The lipid II bands mark the upper boundary of the separating gel. Most of the stacking gel was removed prior to autoradiography. (C and D) SDS-PAGE of M. luteus ATCC 4698 after adsorption of [14C]mersacidin to the cells. (C) Autoradiogram; (D) Coomassie stain of gels run in parallel. Lanes 7 and 12, 5 mg of cells (wet weight); lanes 8 and 13, 2.5 mg of cells; lanes 9 and 14, 1 mg of cells; lane 10, 0.25 nmol of [14C]mersacidin; lane 11, 14C-methylated Rainbow molecular mass marker (Amersham-Buchler).
Article Snippet: Lanes 7 and 12, 5 mg of cells (wet weight); lanes 8 and 13, 2.5 mg of cells; lanes 9 and 14, 1 mg of cells; lane 10, 0.25 nmol of [ 14 C]mersacidin;
Techniques: Isolation, Incubation, Autoradiography, SDS Page, Adsorption, Staining, Methylation, Marker